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KMID : 0545119920020020141
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Journal of Microbiology and Biotechnology
1992 Volume.2 No. 2 p.141 ~ p.151
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Characterization of Physiological Changes in S3H5/γ2bA2 Hybridoma Cells Duing Adaptation to Low Serum Media
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LEE, GYUN MIN
SAVINELL, JOANNE/PALSSON, BERNHARD O.
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Abstract
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Physiological changes of the murine hybridoma cell line S3H5/γ2bA2 during adaptation to RPMI 1640 medium with 1%(v/v) fetal bovine serum were characterized in terms of cell growth, antibody production, morphology, and metabolic quotients. Cells adapted to 1% serum medium in T-flasks became sensitive to shear induced by mechanical agitation and required at least 5% serum in the medium or spent medium for cell growth in spinner flasks, while cells adapted to 10% serum medium in T-flasks could grow in 1% serum medium in spinner flasks. Consequently, long-term adaptation to low serum media may not give the expected growth enhancement. After adaptation to 1% serum medium, changes in cell morphology were observed. The cells in 10% serum medium were uniform and circular, while cells in 1% medium were irregularly shaped. The DNA contents, which were measured by flow cytometry, were almost constant among the cells in the range of 1% to 10%. Further, no significant changes in energy metabolism and specific monoclonal antibody production rate were observed among these cells.
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